鑷�鍣�鏄�閲嶈�佺殑绱�(x矛)鑳炰俊铏熷偝灏�(d菐o)閫氳矾锛岃垏瑷卞�氱敓鐞嗗拰鐥呯悊閬庣▼濡傜��(f膩)鑲�銆佸垎鍖�銆佺�炵��(j墨ng)閫�琛屾�х柧鐥�銆佹噳(y墨ng)婵�銆佹劅鏌撲互鍙婄檶鐧ョ瓑鐩搁棞(gu膩n)銆傚父瑕嬬殑鑷�鍣�鎶戝埗鍔戞槸3-Methyladenine锛�3-MA锛�锛屽畠閫氶亷鎶戝埗PI3K渚嗘姂鍒惰嚜鍣�灏忛珨鐨勫舰鎴�锛屽叾浣跨敤婵冨害姣忔�￠渶mM绱氥��

灞辨澅澶у��(xu茅)瓒欏�剁ゥ鏁欐巿鐮旂��(f膩)鐨勫寲鍚堢墿3BDO鏄�涓�绋�楂樻晥鑳借嚜鍣�鎶戝埗鍔�锛岃垏3-MA鐩告瘮锛�3BDO鍍呴渶μM绱氱殑婵冨害鍗冲彲鎶戝埗鑷�鍣�锛屾槸鐮旂┒鑷�鍣�鐨勬湁鍔涘伐鍏枫�傞櫎姝や箣澶�锛�3BDO閭勬槸鐮旂┒mTOR婵�閰剁殑鏈夋晥宸ュ叿锛屽彲闈跺悜FKBP1A骞舵縺娲�mTOR淇¤櫉閫氳矾锛屾姂鍒�(HUVECs)鑷�鍣�锛堝湒1锛�銆傞倓鍙�浠ヨ垏鍖栧悎鐗�Rapamycin涓�璧蜂綔鐐鸿В鏋愯��(di脿o)鎺х恫(w菐ng)绲�(lu貌)鐨勭爺绌跺伐鍏�^[1-2]銆�

鐧鹃潏濞佽垏瓒欏�剁ゥ鏁欐巿鐛�(d煤)瀹跺悎浣�锛岀偤鎮ㄦ彁渚�3BDO鍖栧悎鐗╋細

• 浣庝娇鐢ㄦ績搴�锛�鑸囨渶甯哥敤鐨勬姂鍒跺姂3-MA锛�mM绱氾級鐩告瘮锛屼綆鑷�μM绱氬嵆鍙�鎶戝埗鑷�鍣�
• 楂樻湁鏁堟��锛�鏈夋晥鎬ф彁鍗�1000鍊�^[1]

鍦�1  3BDO鐮旂┒mTOR婵�閰剁殑鏈夋晥宸ュ叿^[1]

鐝�(xi脿n)璨ㄧ洿渚�锛�

鈻�  3BDO鐢�(ch菐n)鍝�

鈻�  鍏朵粬鐩搁棞(gu膩n)鐢�(ch菐n)鍝�

鈻�  鐢熷寲閫氱敤鑰楁潗绯诲垪

鈻�  鍙冭�冩枃鐛�(xi脿n)锛�

[1] Peng N, et al. Sci Rep. 2014, 4, 5519.

[2] Ge D, et al. Autophagy. 2014, 10(6), 957-971.