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鐢变簬纾烽吀鍖栫殑铔嬬櫧璩�(zh矛)鐩稿皪(du矛)浜庢櫘閫氳泲鐧藉叿鏈変綆璞愬害鍜岄珮鍕�(d貌ng)鎱�(t脿i)鎬х壒榛�(di菐n)锛屽洜姝ゅ皪(du矛)纾烽吀鍖栬泲鐧借唱(zh矛)绲勫(xu茅)妯ｅ搧閫�(j矛n)琛岄珮鐗圭暟鎬у瘜闆�锛屾槸闄嶄綆妯ｅ搧寰�(f霉)闆滅▼搴�銆佹彁楂樼７閰稿寲浣嶉粸(di菐n)閼掑畾鏁�(sh霉)鐩嚦闂�(gu膩n)閲嶈鐨勪竴姝ャ�傚叾涓�锛屽浐瀹氬寲閲戝爆闆㈠瓙瑕拰鑹茶瓬娉曪紙IMAC锛夋槸纾烽吀鍖栬泲鐧借唱(zh矛)绲勫(xu茅)鐮旂┒涓渶甯哥敤鐨勭７閰稿寲鑲芥瀵岄泦鎶�琛�(sh霉)涔嬩竴銆�
鐧鹃潏濞佹敎鎵嬩腑绉戦櫌澶у寲鎵�钁夋槑浜暀鎺�锛屾彁渚涙柊涓�浠ｇ７閰歌偨瀵岄泦鏉愭枡——Ti-IMAC[1]锛岀敱浜庡叾鍗撹秺鐨勫瘜闆嗘�ц兘锛屽湪鍦�(gu贸)鍏�(n猫i)澶栫嵅寰椾簡寤ｆ硾闂�(gu膩n)娉ㄣ�傜洰鍓�锛屽凡鏈�70澶氬��(g猫)鍦�(gu贸)鍏�(n猫i)澶栫煡鍚嶉珮鏍°�佺鐮旈櫌鎵�銆佺敓鐗╃鎶�鍏徃浣跨敤鎴戝徃Ti-IMAC鏉愭枡銆�Ti-IMAC鏉愭枡琚噳(y墨ng)鐢ㄤ簬浜鸿倽纾烽吀鍖栬泲鐧借唱(zh矛)绲勭殑瑕�(gu墨)?锛涱嚞娌�?锛屽緱鍒颁簡浜鸿倽绲勭箶鏈�澶х殑纾烽吀鍖栦綅榛�(di菐n)鏁�(sh霉)鎿�(j霉)闆嗭紙涓嬪湒1锛�銆�[2]
鈻� 鐢�(ch菐n)鍝佺壒鎬�
- 瓒呴珮鐨勮泲鐧借唱(zh矛)纾烽吀鍖栭憭瀹氳兘鍔涳細鏄父瑕�(gu墨)鍥哄畾鍖栭噾灞洟瀛愯Κ鍜岃壊璀滄硶锛�Fe3+-IMAC锛夌殑3鍊嶄互涓�锛岃垏TiO2鐩告瘮鍙互鎻愰珮绱�30%锛屽瘜闆嗙壒鐣版�у彲閬�(d谩)95%[3]锛�
- 鏇撮珮鐨勭７閰歌偨瀵岄泦鏁堢巼锛氳嵎铇湅(gu贸)瀹惰泲鐧借唱(zh矛)绲勫(xu茅)鐮旂┒涓績鐨�Heck鏁欐巿瀵�(sh铆)椹�(y脿n)瀹ゅ皥闁�灏�(du矛)瑭叉潗鏂欓��(j矛n)琛屼簡娓�(c猫)瑭�锛岀祼(ji茅)鏋滆〃鏄庤垏甯歌(gu墨)鐨勭７閰歌偨瀵岄泦鏉愭枡濡�TiO2鐩告瘮锛�Ti-IMAC鍏锋湁鏇撮珮鐨勫瘜闆嗘晥鐜�[4]锛�
- 鐛�(d煤)瀹剁従(xi脿n)璨ㄤ緵鎳�(y墨ng)銆�
鍦�1 Ti-IMAC琚噳(y墨ng)鐢ㄤ簬浜鸿倽纾烽吀鍖栬泲鐧借唱(zh矛)绲勭殑瑕�(gu墨)妯″寲鍒嗘瀽锛屽緱鍒颁簡浜鸿倽绲勭箶鏈�澶х殑纾烽吀鍖栦綅榛�(di菐n)鏁�(sh霉)鎿�(j霉)闆�[2]
鈻� 鐢�(ch菐n)鍝佸垪琛�
鈻� 鍏朵粬鐩搁棞(gu膩n)鐢�(ch菐n)鍝�
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鐢�(ch菐n)鍝佺法铏�(h脿o)
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鐢�(ch菐n)鍝佸悕绋�
|
CAS铏�(h脿o)
|
绱斿害
|
鍖呰瑕�(gu墨)鏍�
|
|
140105
|
1,4-Butanediol diglycidyl ether, 95%
1,4-涓佷簩閱囦簩绺按鐢樻补閱�
|
2425-79-8
|
95%
|
25g
100g
500g
|
|
970232
|
4-Aminobenzamidine dihydrochloride
4-姘ㄥ熀鑻敳鑴掍簩楣介吀楣�,瓒呯磾98%
|
2498-50-2
|
98%
|
1g
5g
25g
100g
|
|
314162
|
Sodium cyanoborohydride, 95%
姘板熀纭兼矮鍖栭垑
|
25895-60-7
|
95%
|
10g
50g
250g
|
|
551313
|
3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester
3-(2-鍚″暥浜岀～浠�)涓欓吀-N-鐞ョ弨閰颁簽鑳洪叝
|
68181-17-9
|
96%
|
100mg
|
|
308310
|
Streptavidin
閺堥湁瑕拰绱�
|
9013-20-1
|
N/A
|
5mg
25mg
|
|
249096
|
2-Mercaptoethanol, 99%, for electrophoresis and molecular biology
2-宸板熀涔欓唶
|
60-24-2
|
99%
|
10ml
250ml
|
|
191243
|
N,N'-Disuccinimidyl carbonate, 99%
N,N'-浜岀惀鐝�閰颁簽鑳哄熀纰抽吀閰�
|
74124-79-1
|
99%
|
5g
25g
100g
|
|
183144
|
N-Hydroxysulfosuccinimide sodium salt, 98%
N-缇ュ熀纾�?锛涱嫎顚剁壘顥嗗暫灏乧楣�
|
106627-54-7
|
98%
|
250mg
1g
5g
|
|
226162
|
Tris(hydroxymethyl)aminomethane
涓�(缇ョ敳鍩�)姘ㄥ熀鐢茬兎锛岃秴绱�
|
77-86-1
|
99.5%
|
25g
100g
500g
2.5kg
|
|
910322
|
mPEG-Amine, 95%, average M.W. 5,000
鐢叉哀鍩�-PEG-鑳�
|
80506-64-5
|
95%
|
1g
5g
|
|
932987
|
Amine-PEG-Amine, 95%, average M.W. 2,000
姘ㄥ熀-PEG-鑳�
|
24991-53-5
|
95%
|
1g
5g
|
鈻� 鐩搁棞(gu膩n)鐢�(ch菐n)鍝�
鈻�鍙冭�冩枃鐛�(xi脿n)
[1] Zhou H, Ye M, Dong J, Han G, Jiang X, Wu R, Han F. Journal of Proteome Research. 2008, 7, 3957-3967.
[2] Bian Y, et al. J. Proteomics. 2014, 96, 253-262.
[3] Yao Y, Dong J, Dong M, Liu F, Wang Y, Mao J, Ye M, Zou H. J. Chromatogr. A. 2017, 1498, 22–28.
[4] Zhou, Houjiang,Low, Teck Y.,Hennrich, Marco L.,et al. Molecular & Cellular Proteomics. 2011,10(10):1.
鈻� 閰嶅瑭﹀姂瑾�(shu艒)鏄�1
銆愮敘(ch菐n)鍝佺法铏�(h脿o)銆�2749380
銆愯嫳鏂囧悕绋便��CAE-Ti-IMAC, 100%
銆愪腑鏂囧悕绋便�戝柈鍒嗘暎鍥哄畾鍖栬Κ鍜岃壊璀滃井鐞�
銆愮敘(ch菐n)鍝佺啊(ji菐n)浠嬨�戞湰鐢�(ch菐n)鍝侀仼鐢ㄤ簬灏�(du矛)纾烽吀鑲界殑閼掑畾鍜屽瘜闆�銆�
銆愪娇鐢ㄦ鐢�(ch菐n)鍝佹墍闇�瑭﹀姂鍒楄〃銆�
|
瑭﹀姂鍒楄〃
|
涓枃鍚嶇ū
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鎵�闇�婧惰唱(zh矛)
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CAS
|
灏�(du矛)鎳�(y墨ng)鎴戝徃鐢�(ch菐n)鍝佺法铏�(h脿o)
|
|
Ti(SO4)2
|
纭吀閳�
|
纭吀閳�
|
13693-11-3
|
鐒�(w煤)
|
|
0.1% TFA姘存憾娑�
|
0.1%涓夋盁涔欓吀姘存憾娑�
|
涓夋盁涔欓吀
|
76-05-1
|
252705
|
|
200mM NaCl姘存憾娑�
|
200mM 姘寲閳夋按婧舵恫
|
姘寲閳�
|
7647-14-5
|
105864
|
|
30% H2O2
|
30% 闆欐哀姘�
|
30% 闆欐哀姘�
|
7722-84-1
|
鐒�(w煤)
|
|
80%ACN
|
80%涔欒厛
|
涔欒厛
|
75-05-8
|
168171
|
|
6% TFA姘存憾娑�
|
6%涓夋盁涔欓吀姘存憾娑�
|
涓夋盁涔欓吀
|
76-05-1
|
252705
|
|
50%ACN
|
50%涔欒厛
|
涔欒厛
|
75-05-8
|
168171
|
|
10%姘ㄦ按婧舵恫
|
10%姘ㄦ按婧舵恫
|
10%姘ㄦ按婧舵恫
|
7664-41-7
|
鐒�(w煤)
|
|
1%FA姘存憾娑�
|
1%鐢查吀姘存憾娑�
|
鐢查吀
|
64-18-6
|
949722
|
鈻� 閰嶅瑭﹀姂瑾�(shu艒)鏄�2
銆愮敘(ch菐n)鍝佺法铏�(h脿o)銆�2749381
銆愯嫳鏂囧悕绋便��SPE-Ti-IMAC, 100%
銆愪腑鏂囧悕绋便�戝浐鐩歌悆鍙栧浐瀹氬寲瑕拰鑹茶瓬寰悆
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灏�(du矛)鎳�(y墨ng)鎴戝徃鐢�(ch菐n)鍝佺法铏�(h脿o)
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|
Ti(SO4)2
|
纭吀閳�
|
纭吀閳�
|
13693-11-3
|
鐒�(w煤)
|
|
0.1% TFA姘存憾娑�
|
0.1% 涓夋盁涔欓吀姘存憾娑�
|
涓夋盁涔欓吀
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76-05-1
|
252705
|
|
200mM NaCl姘存憾娑�
|
200mM 姘寲閳夋按婧舵恫
|
姘寲閳�
|
7647-14-5
|
105864
|
|
30% H2O2
|
30% 闆欐哀姘�
|
30% 闆欐哀姘�
|
7722-84-1
|
鐒�(w煤)
|
|
80%ACN
|
80%涔欒厛
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75-05-8
|
168171
|
|
6% TFA姘存憾娑�
|
6%涓夋盁涔欓吀姘存憾娑�
|
涓夋盁涔欓吀
|
76-05-1
|
252705
|
|
50%ACN
|
50%涔欒厛
|
50%涔欒厛
|
75-05-8
|
168171
|
|
10%姘ㄦ按婧舵恫
|
10%姘ㄦ按婧舵恫
|
10%姘ㄦ按婧舵恫
|
7664-41-7
|
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|
|
1%FA姘存憾娑�
|
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64-18-6
|
949722
|
|
200 μL妲嶉牠
|
绉绘恫鍣ㄥ惛闋�
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|
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|
970956
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|
600 ul闆㈠績绠�
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闆㈠績绠�
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|
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910854
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|
1.5 ml闆㈠績绠�
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闆㈠績绠�
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915664
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|
SPE鏌辩鍙婄鏉�
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绡╂澘
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1ml锛�2579375
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