鍦ㄩ叾鍏嶇柅鍒嗘瀽(EIA)涓�锛屼竴绋叾鑳藉偓鍖栫壒瀹氱殑搴曠墿, 灏囧叾杞�(zhu菐n)鍖栫偤鏈夊彲妾㈡脯淇¤櫉(h脿o)鐨勭敘(ch菐n)鐗╋紝瑭叉妧琛�(sh霉)鏄敓鐗╅啱(y墨)钘ュ熀绀�(ch菙)鐮旂┒銆佽嚚搴婃椹�(y脿n)鍜岃(w猫i)鐢熸椹�(y脿n)鐨勯棞(gu膩n)閸垫妧琛�(sh霉)銆傚偝绲�(t菕ng)鐨凟IA閲囩敤鑹插師搴曠墿娓噺鍙鍏夌殑鍚稿厜搴�, 闅ㄨ憲閰跺厤鐤垎鏋愭妧琛�(sh霉)鐨勭櫦(f膩)灞曪紝涓嶆柗鍜岀啋鍏�銆佺櫦(f膩)鍏夋妧琛�(sh霉)浠ュ強鍎�鍣ㄨ嚜鍕�(d貌ng)鍖栫浉铻嶅悎锛屾棩鑷诲畬鍠�[3]銆傝繎骞惧勾浠ヤ締锛岀磵绫虫潗鏂欓枊濮嬩綔鐐虹敓鐗╃磵绫虫ā鎿叾琚唬娉涚爺绌躲�俌an 绛�[4]鐧�(f膩)鐝�(xi脿n) Fe3O4纾佹�х磵绫抽绮掑彲浠ュ偓鍖朒2O2姘у寲閬庢哀鍖栫墿閰跺簳鐗� 3,3’,5,5’-鍥涚敳鍩鸿伅(li谩n)鑻兒(TMB)銆侀噸姘皑鍩鸿嫰(DAB)鍜岄劙鑻簩鑳�(OPD)鐧�(f膩)鐢熼鑹茶畩鍖栥�� 鑸囧ぉ鐒堕叾妾㈡脯鐩告瘮锛屽叿鏈夌┅(w臎n)瀹氬拰鎴愭湰浣庣殑鍎�(y艒u)榛�(di菐n)銆傜櫨闈堝▉浣滅偤灏堟キ(y猫)瑭﹀姂渚涙噳(y墨ng)鍟嗭紝鎻愪緵甯哥敤閰跺垎鏋愭娓簳鐗╁拰鐢熺墿绱嶇背鏉愭枡锛� 鍖呮嫭杈ｆ牴閬庢哀鍖栫墿閰跺簳鐗�銆佸牽鎬х７閰搁叾搴曠墿銆�β-D-鍗婁钩绯栬嫹閰跺簳鐗�銆侀噾灞磵绫抽绮掋�佺磵绫冲井鐞�銆佺⒊绱嶇背绠＄瓑銆�
- 鐢�(ch菐n)鍝侀潏鏁忓害楂�
- 鏄撲簬閰嶇疆淇濆瓨锛岀┅(w臎n)瀹氭�у挤(qi谩ng)
- 绋澶氭ǎ锛岀従(xi脿n)璨ㄥ韩瀛�
鍦� 1锛歛 鍚湁GSH鐨勬娓(j墨)鐞嗙ず鎰忓湒锛宐 涓嶅惈鏈塆SH鐨勬娓(j墨)鐞嗙ず鎰忓湒[1]銆�
.png)
鍦� 2锛欶e3O4绱嶇背椤嗙矑鍌寲姘у寲涓夌ó閰跺簳鐗� TMB, DAB 鍜� OPD 鐧�(f膩)鐢熶笉鍚岀殑椤忚壊璁婂寲绀烘剰鍦�[4]銆�
鍦� 3锛氭噳(y墨ng)鐢ㄥ厜鍌寲鐨勭～鍖栭帢绱嶇背绮掑瓙妾㈡脯钁¤悇绯栨哀鍖栭叾鍜岃胺鑳辩敇鑲介倓鍘熼叾[2]銆�
References
[1] Ni P, Sun Y, Dai H, et al. Highly sensitive and selective colorimetric detection of glutathione based on Ag[I] ion–3,3′,5,5′-tetramethylbenzidine (TMB)[J]. Biosensors & Bioelectronics, 2014, 63C:47-52.
[2] Grinyte R, Garai-Ibabe G, Saa L, et al. Application of photocatalytic cadmium sulfide nanoparticles to detection of enzymatic activities of glucose oxidase and glutathione reductase using oxidation of 3,3′,5,5′-tetramethylbenzidine[J]. Analytica Chimica Acta, 2015, 881:131-138.
[3] Lin Lin, Dongmin Shi, Quanfa Li,绛�. Detection of T4 polynucleotide kinase based on a MnO2 nanosheet-3,3′,5,5′-tetramethylbenzidine (TMB) colorimetric system[J]. Analytical Methods, 2016, 8(20):4119-4126.
[4] 鏉庨簵楹�. 鍩轰簬寰磵绫虫潗鏂欓亷姘у寲鐗╂ā鎿叾鐗规�х殑鐢熺墿鍌虫劅鍣ㄧ爺绌禰D]. 瑗胯彲甯寖澶у(xu茅), 2015.
鈻� 閬庢哀鍖栫墿閰跺簳鐗�
|
鐢�(ch菐n)鍝佺法铏�(h脿o)
|
鐢�(ch菐n)鍝佸悕绋�
|
CAS
|
鍖呰瑕�(gu墨)鏍�
|
|
602529
|
4-Aminoantipyrine, 98%
4-姘ㄥ熀瀹夋浛姣旀灄, 98%
|
83-07-8
|
25 g
100 g
500 g
|
|
213644
|
3-Dimethylaminobenzoic acid, 99%
3-浜岀敳姘ㄥ熀鑻敳閰�, 99%
|
99-64-9
|
25 g
100 g
500 g
|
|
236213
|
5-Aminosalicylic acid, 98.5%
5-姘ㄥ熀姘存閰�, 98.5%
|
89-57-6
|
5 g
25 g
100 g
|
|
185223
|
2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt/ABTS, 98%
2,2'-鑱�(li谩n)姘洐(3-涔欏熀鑻苟鍣诲攽鍟�-6-纾洪吀)浜岄姩楣�, 98%
|
30931-67-0
|
1 g
5 g
25 g
|
|
504147
|
3,3'-Dimethoxybenzidine dihydrochloride, 99%
3,3'-浜岀敳姘у熀鑱�(li谩n)鑻兒浜岄菇閰搁菇, 99%
|
20325-40-0
|
5 g
25 g
100 g
|
|
292086
|
3,3',5,5'-Tetramethylbenzidine dihydrochloride, 98%
3,3',5,5'-鍥涚敳鍩鸿伅(li谩n)鑻兒浜岄菇閰搁菇, 98%
|
64285-73-0
|
1 g
5 g
25 g
|
|
433886
|
3-(4-Hydroxyphenyl)propionic acid, 99%
3-(4-缇ヨ嫰鍩�)涓欓吀, 99%
|
501-97-3
|
5 g
25 g
100 g
|
|
293137
|
7-Hydroxycoumarin-3-carboxylic acid ethyl ester, 98%
7-缇ュ熀棣欒眴绱�-3-缇ч吀涔欓叝, 98%
|
6093-71-6
|
25 mg
500 mg
1 g
|
鈻� 鍫挎�х７閰搁叾搴曠墿
|
鐢�(ch菐n)鍝佺法铏�(h脿o)
|
鐢�(ch菐n)鍝佸悕绋�
|
CAS
|
鍖呰瑕�(gu墨)鏍�
|
|
132283
|
Adenosine, 99%
鑵鸿嫹, 99%
|
58-61-7
|
5 g
25 g
100 g
|
|
545833
|
4-Nitrophenyl β-D-lactopyranoside, 98%
4-纭濆熀鑻熀 β-D-鍚″杻涔崇硸鑻�, 98%
|
4419-94-7
|
50 mg
100 mg
|
|
141887
|
5-Bromo-4-chloro-3-indolyl phosphate disodium salt, 98%
5-婧�-4-姘�-3-鍚插摎纾烽吀浜岄垑楣�, 98%
|
102185-33-1
|
50 mg
250 mg
1 g
|
|
150020
|
5-Bromo-6-chloro-3-indolyl phosphate p-toluidine salt, 98%
(5-婧�-6-姘�-3-鍚插摎鍩�)纾烽吀灏嶇敳鑻兒楣�, 98%
|
6769-80-8
|
50 mg
250 mg
|
|
472252
|
o-Dianisidine, 98%
閯拌伅(li谩n)鑼撮鑳�, 98%
|
119-90-4
|
5 g
25 g
100 g
|
|
254303
|
p-Nitrophenyl phosphate disodium salt hexahydrate, 98%
灏嶇鍩鸿嫰纾烽吀浜岄垑楣藉叚姘村悎鐗�, 98%
|
333338-18-4
|
1 g
5 g
25 g
|
|
1084029
|
4-Nitrophenyl phosphate disodium salt hexahydrate, 98%
4-纭濆熀鑻熀纾烽吀浜岄垑楣藉叚姘村悎鐗�, 98%
|
4264-83-9
|
1 g
5 g
25 g
|
|
272074
|
Phenolphthalein monophosphate di(cyclohexylammonium) salt, 90%
閰氶厼鍠７閰搁叝闆欑挵(hu谩n)宸卞熀閵ㄩ菇, 90%
|
14815-59-9
|
1 g
5 g
|
|
239270
|
α-Ketoglutaric acid, 99%
α-閰垔浜岄吀, 99%
|
328-50-7
|
25 g
100 g
500 g
|
鈻� β-鍗婁钩绯栬嫹閰跺簳鐗�
|
鐢�(ch菐n)鍝佺法铏�(h脿o)
|
鐢�(ch菐n)鍝佸悕绋�
|
CAS
|
鍖呰瑕�(gu墨)鏍�
|
|
440990
|
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, 99%
5-婧�-4-姘�-3-鍚插摎鍩� β-D-鍚″杻鍗婁钩绯栬嫹, 99%
|
7240-90-6
|
100 mg
1 g
|
|
184406
|
2-Chloro-4-nitrophenyl β-D-galactopyranoside, 98%
2-姘�-4-纭濆熀鑻熀 β-D-鍚″杻鍗婁钩绯栬嫹, 98%
|
123706-60-5
|
1 g
|
|
247160
|
4-Nitrophenyl β-D-galactopyranoside, 98%
4-纭濆熀鑻熀 β-D-鍚″杻鍗婁钩绯栬嫹, 98%
|
3150-24-1
|
1 g
5 g
|
|
446877
|
2-Nitrophenyl β-D-galactopyranoside, 98%
5-婧�-4-姘�-3-鍚插摎鍩� β-D-鍚″杻鍗婁钩绯栬嫹, 98%
|
369-07-3
|
100 mg
1 g
|
鈻� 鐔掑厜閰跺簳鐗�
|
鐢�(ch菐n)鍝佺法铏�(h脿o)
|
鐢�(ch菐n)鍝佸悕绋�
|
CAS
|
鍖呰瑕�(gu墨)鏍�
|
|
433521
|
D-Luciferin potassium salt, 98%
D-鐔掑厜绱犻墍楣�, 98%
|
115144-35-9
|
5 mg
25 mg
100 mg
|
|
308290
|
D-Luciferin sodium salt, 98%
D-鐔掑厜绱犻垑楣�, 98%
|
103404-75-7
|
5 mg
25 mg
100 mg
|
|
106094
|
3-Aminophthalhydrazide monosodium salt, 98%
3-姘ㄥ熀閯拌嫰浜岄叞鑲煎柈閳夐菇, 98%
|
20666-12-0
|
1 g
5 g
25 g
|
|
393715
|
5-Fluoroorotic acid hydrate, 98%
5-姘熶钩娓呴吀姘村悎鐗�, 98%
|
703-95-7
|
100 mg
1 g
5 g
|
鈻� 鐩搁棞(gu膩n)鐢�(ch菐n)鍝侊細